Skip to main content

DNA Profiling

DNA Profiling: enhancing the control of Crime through emerging technologies

 How crime can be controlled by specific Biotechnology Applications of DNA Fingerprinting?

DNA. i.e.Deoxyribose Nucleic Acid acts as genetic material. The unique property of the DNA due to its chemical properties. The chemical structure of everyone's DNA is the same but the only difference between person DNA is the order of the base pairs. Hence the result is every person could be identified solely by its sequence of their base pairs. 

DNA profiling is a technique used to distinguish between individuals of the same species using only samples of their DNA. This is a method used to identify an individual from a sample of DNA with the help unique pattern that is present in their DNA.

The original DNA fingerprinting technique was developed by Alec Jeffreys in 1985. DNA Profiling knew as DNA testing, DNA typing, genetic fingerprinting, and DNA fingerprinting.DNA fingerprint is an analysis of the nitrogenous base sequence in the DNA of an individual.

(DNA Fingerprint is a Molecular marker pattern of an individual obtained by using highly polymorphic markers; used for unequivocal identification of strains, individuals, criminals, etc.)

The structure of each person’s genome is unique. The unique nature of genome structure provides a good opportunity for the specific identification of an individual.

(*The only exception in the genome structure being monozygotic identical twins because in this case, twins developed from a single fertilized ovum)

DNA profiling exploits highly variable repeat sequences called VNTRs( Variable Number Tandem Repeats).VNTRs generally include micro-satellites and mini-satellites.Within the specific sequence of the repeat units composing VNTR are highly conserved among individuals. Differences in the number of repeats thus in the length of a simple -sequence e tandem array containing the same repeat units, are quite common among individuals. These DNA polymorphism firm the basis of DNA fingerprinting. The high variability in minisatellites makes them useful for genomic mapping because there is a high probability that individuals will vary in their alleles at such a locus.

[* VNTRs are located in a particular site on a pair of homologous chromosomes.VNTRs consists of a core sequence that is repeated several times. VNTRs identified two types: one is Mini-satellites ( 20–100 bases repeated 0–30 times, and the second one is 2–5 bases repeated 5–50 times this type of micro-satellite also called Short Tandem Repeats(STRs).VNTRs commonly found ]in the introns .ie. non-coding region.]


  1. Sample of DNA is first amplified by using PCR .i.e. Polymerase Chain Reaction.
  2. Restriction enzymes are used to cut the DNA at different sizes because different people have different sequences. So, restrictions enzymes help to get a required size of DNA. This shows a set of different-sized fragments unique to the individuals.
  3. These fragments then separate according to the size with the help of gel electrophoresis. 
  4. Probe with label sequences is used to see the fragments of DNA.


  1. Paternity

DNA profiling is used for the settlement of paternity disputes. This technique helps to determine whether or not an individual is the parent of a child and helps to establish the true parents. 

2.Forensic Science 

This DNA Profiling is used to compared the sample collected from the crime scene with suspects. That helps to establish beyond a reasonable doubt that a person is guilty of a crime for which he or she has been accused.

3.Food safety and security

DNA profiling plays an important role to detect food safety and food security that help for the identification and parentage of plants and animals. 

4.Diagnosis of genetic disease

DNA profiling plays a very important role in genetic research. This technique is applied to identify, isolate, and develop to cure genetic diseases. 

DNA profiling is the present-day genetic detective in the practice of modern medical forensics. 

Image References:


  1. I liked your work and the way in which you have shared this article here about pregnancy scans Perth. It is a beneficial and helpful article for us. Thanks for sharing an article like this.


Post a Comment

Please do not enter any spam link in the comment box.

Popular posts from this blog

Red Biotechnology

  20 Major Applications Of Red Biotechnology Red Biotechnology is a branch of modern biotechnology T he term Biotechnology was introduced by a Hungarian engineer, Karl Ereky in 1917. Ereky defined biotechnology as “ all lines of work by which products are produced from raw materials with the aid of living things”. Biotechnology is a multidisciplinary application. Biotechnological research has a wide range of applications that are shown in medical and health science, agriculture science, and environmental science. Bio-Pharmaceutical is known as Red- Biotechnology. This branch of biotechnology is related to health care. The main utilization of this branch is shown in the field of medicine. Red biotechnology helps to improve the quality of life. Red-Biotechnology has a wide range of applications. Many applications are shown in medical and health science, genetic engineering, gene therapy, and many more fields related to the medical line. 20 Major Applications of Red Biotechnology are: 1.

Green biotechnology

  17 Most Important Applications Of Green biotechnology Agrobiotechnology  Green biotechnology related to plants of improving both the quality and quantity of plants. The main goal of green biotechnology is the production of an improved variety of plants, with the help of biotechnology-based techniques that should be environmentally friendly solutions.  The main aim of green biotechnology is to tackle food security issues and reduce the environmental footprint of the industry. Green biotechnology can improve the global food situation.  17 Most Important Applications Of Green Biotechnology :  1. Micropropagation  One of the most important applications of green biotechnology is the micropropagation of plant species. this technique has attained the status of a large plant-based industry. Micropropagation is the true type of propagation of selected genotypes using small pieces of tissues which can stay as an important technique for rapid production of many commercially important plants lik

20 enzymes that are used in the food & Beverages industry

  20 enzymes that are used in the food & Beverages industry Enzymes name and its uses E nzymes are biological molecules typically made up of proteins, it is a fundamental task of proteins. The enzyme is very sensitive in nature. many physiochemical changes in the enzyme are affecting by changes in pH, temperature, substrate concentration, etc.  The enzyme has a wide range of applications. There are several uses of an enzyme in the biofuel industry, biological detergent, culinary uses, dairy industry, molecular biology, paper industry, personal care, starch industry, food production, food processing, and food preservation.  As per recent estimates, a great majority of industrially produced enzymes are useful in processes related to foods(45%), detergents (35%), textiles ( 10%), and leather(3%).   An enzyme is a biocatalyst that increases the rate of a chemical reaction without itself undergoing any change, Virtually all cellular reactions are mediated by the enzyme. The enzyme has s