Kiranjeet Kaur Biotechnologist

  List of 17 Enzymes with their function that are used in recombinant DNA technology /Genetic Engineering     1.      Alkaline phosphatase : This enzyme helps to remove the phosphate groups from 5’-ends of double and single standard DNA and RNA. It also helps prevent re-circularization during cloning and dephosphorylate DNA before Kinase labeling protocols. 2.   Terminal Transferase : Enzyme very useful in homopolymer tailing. It helps to add nucleotides to the 3’ends of DNA or RNA. This enzyme is used as a template for a primer in subsequent PCR. 3.      Bal 31 nuclease : In DNA technology this enzyme is used to degrade both 3’ and 5’ termini of duplex DNA without generating any internal scissions. In short, this enzyme is used for the progressive shortening of DNA. 4.     S1nuclease : It is an endonuclease enzyme that helps to degrade single-standard DNA and RNA but can’t degrade double-standard D...

  20 Major Applications Of Red Biotechnology Red Biotechnology is a branch of modern biotechnology T he term Biotechnology was introduced by a Hungarian engineer, Karl Ereky in 1917. Ereky defined biotechnology as “ all lines of work by which products are produced from raw materials with the aid of living things”. Biotechnology is a multidisciplinary application. Biotechnological research has a wide range of applications that are shown in medical and health science, agriculture science, and environmental science. Bio-Pharmaceutical is known as Red- Biotechnology. This branch of biotechnology is related to health care. The main utilization of this branch is shown in the field of medicine. Red biotechnology helps to improve the quality of life. Red-Biotechnology has a wide range of applications. Many applications are shown in medical and health science, genetic engineering, gene therapy, and many more fields related to the medical line. 20 Major Applications of Red Biotechnology are...

  3 Blotting Techniques used for the identification of Nucleic Acid Basics techniques in the genetic engineering field                               Image Credit to Polina Tankilevitch /Unsplash  Nucleic acid like DNA and RNA is widely used in various research work. So, the identification of desired DNA and RNA from thousands of molecules becomes the basic requirement. The blotting technique is widely used as an analytical tool for the identification of desired DNA and RNA fragments. The basic principle behind this blotting technique is the process based on immobilization of samples i.e. nucleic acid and solid support.  Blot helps to transfer nucleic acid onto the carrier so they can easily be separated from the desired nucleic acid fragment. The solid support or carrier mostly used in the blotting technique are Nitrocellulose, Nylon membrane, and DBM paper that helps for the blot nucleic acid...

  Artificial Active Immunity  Immunization or Vaccination  Image Credit to Karolina Grabowska on pexels.com S everal diseases show their effects. Our immune system is so weak due to our living style and eating habits. our body does not respond properly against the pathogens due to the weak immune systems. So, scientists invent a vaccine. When naturally our body resists to respond or produce antibodies against these harmful pathogens then artificial active immunity is provided via a vaccination. The early vaccine process for immunization based on the idioms —  Diamond Cut Diamond means to say those disease effects by a pathogen, the method to kill or weakens the effect of this pathogen produces vaccines from the same or related pathogen by weakens its pathogenicity. For example -To immunize the snake bite the vaccine is prepared from the snake venom. The vaccine also an immunogen helps to induce artificial active immunity. The vaccine is a mixture of an antigen that h...