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Showing posts with the label Southern blotting

Blotting Technique used for Identification of Nucleic Acid

  3 Blotting Techniques used for the identification of Nucleic Acid Basics techniques in the genetic engineering field                               Image Credit to Polina Tankilevitch /Unsplash  Nucleic acid like DNA and RNA is widely used in various research work. So, the identification of desired DNA and RNA from thousands of molecules becomes the basic requirement. The blotting technique is widely used as an analytical tool for the identification of desired DNA and RNA fragments. The basic principle behind this blotting technique is the process based on immobilization of samples i.e. nucleic acid and solid support.  Blot helps to transfer nucleic acid onto the carrier so they can easily be separated from the desired nucleic acid fragment. The solid support or carrier mostly used in the blotting technique are Nitrocellulose, Nylon membrane, and DBM paper that helps for the blot nucleic acid...