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List of 17 Enzymes with their function that are used in recombinant DNA technology /Genetic Engineering

  List of 17 Enzymes with their function that are used in recombinant DNA technology /Genetic Engineering     1.      Alkaline phosphatase : This enzyme helps to remove the phosphate groups from 5’-ends of double and single standard DNA and RNA. It also helps prevent re-circularization during cloning and dephosphorylate DNA before Kinase labeling protocols. 2.   Terminal Transferase : Enzyme very useful in homopolymer tailing. It helps to add nucleotides to the 3’ends of DNA or RNA. This enzyme is used as a template for a primer in subsequent PCR. 3.      Bal 31 nuclease : In DNA technology this enzyme is used to degrade both 3’ and 5’ termini of duplex DNA without generating any internal scissions. In short, this enzyme is used for the progressive shortening of DNA. 4.     S1nuclease : It is an endonuclease enzyme that helps to degrade single-standard DNA and RNA but can’t degrade double-standard DNA or RNA-DNA hybrids. 5.      DNA Ligase : In DNA replication, the DNA